MAbPac SEC– 1 for Monoclonal Antibody (MAb) Analysis
MAbPac? SEC-1 is a size exclusion chromatography (SEC) column designed for
monoclonal antibody (MAb) analysis, including monomers, aggregates, and
fragments, under non-denaturing conditions, and in both high- and
low-salt mobile phases and volatile eluents.
? Proprietary
hydrophilic bonded layer results in minimal non-desired interactions
between the biomolecules and the stationary phase.
? Nonmetallic and Biocompatible PEEK? housing eliminates metal contamination from the column hardware.
? Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and Corona? CAD detection.
? Rugged, reproducible column.
? Superior performance for the analysis of monoclonal antibodies, even at low salt concentrations.
Applications
SEC
is frequently used because of its simple operating principle and
features not found in other separation techniques including separating
aggregates that are not easily distinguished by other chromatographic
means. MAbs produced from mammalian cell culture may contain significant
amounts of dimers and higher-order aggregates. The MAbPac SEC-10 is a
high-resolution SEC column that is suitable for use with a wide range of
biomolecules, including monoclonal antibodies and their aggregates.
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MAbPac? SEC-1 Monoclonal Antibody Analysis Column Specifications
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Column Dimension
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4 × 300 mm, 4 × 50 mm, PEEK? housing
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Particle Size
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5 μm
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Bonding Chemistry
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Proprietary diol
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Silica Substrate
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Spherical, high-purity porous silica
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Pore size
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300 ?
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Flow Rate Range:
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0.2 to 0.3 mL/min.
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Pressure Limit
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1800 psi
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Temperature Range:
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Ambient to 25 oC
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pH Range
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2.2–7.5
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Typical buffers:
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50 mM phosphate buffer (pH 6.8) + 0.3 M NaCl,
PBS and other commonly used buffers
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Solvent Compatibility:
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Compatible with 100% organic solvents
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Separation range for globular proteins
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10,000–1,000,000
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Exclusion limit for globular proteins
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>1,000,000
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Detergent Compatibility
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Compatible with 0.1% SDS (though binding will be irreversible and
it is recommended that the column be dedicated to this application)
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The MAbPac SEC-1 column is based on high-purity, spherical, porous
(300 ?), 5 μm silica particles covalently modified with a proprietary
diol hydrophilic layer. The column is packed into nonmetallic,
biocompatible, PEEK column housing to eliminate compromised
chromatographic due to metal from the column hardware.
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Analytical Columns
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MAbPac SEC-1, 5μm, 300?, analytical column PEEK (4.0 x 300mm)
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074696
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MAbPac SEC-1, 5μm, 300?, analytical column PEEK (4.0 x 150mm)
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075592
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Guard Columns
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MAbPac SEC-1, 5μm, 300?, guard column PEEK (4.0 x 50mm)
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074697
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