美國戴安Dionex Acclaim 300 C18 Column 164718 生物色譜柱
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產品名稱: 美國戴安Dionex Acclaim 300 C18 Column 164718 生物色譜柱
產品型號: 164718
產品展商: 戴安Dionex
產品文檔: 無相關文檔
簡單介紹
戴安公司生產的儀器主要有離子色譜儀、高效液相色譜儀、快速溶劑萃取儀和氨基酸直接分析儀。其中離子色譜儀憑借著雄厚的技術優勢和方法已廣泛地應用于各個領域,其市場占有率達80%。全球與離子色譜相關的文章有90%均出自戴安公司生產的離子色譜儀。
戴安公司的快速溶劑萃取儀15分鐘之內僅用15毫升溶劑既可完成對10g樣品的萃取,該技術已在環境、**、食品和聚合物工業等領域得到廣泛應用。
美國戴安Dionex Acclaim 300 C18 Column 164718 生物色譜柱
的詳細介紹
Acclaim 300 C18 Protein and Peptide Column
High-Resolution Separation of Proteins and Peptides
The Acclaim? 300 features 3 μm silica particles for rapid analysis of complex
protein digests. The Acclaim 300’s stable bonding make these columns
compatible with a wide variety of applications, including LC/MS methods.
-
Designed for high-resolution peptide mapping and protein separations
-
High-efficiency 3 μm spherical silica substrate
-
High-performance bonding chemistry on 300 ? pore silica
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Application tested for suitablility in peptide mapping
-
Reproducible for dependable results
-
LC/MS compatible
Manufacture
of these columns starts with ultrapure silica that contains minimal
concentrations of metal contaminants, minimizing the tailing effects of
residual, exposed silanol groups. Exhaustive bonding and end-capping
techniques result in stable bonding and columns that exhibit predictable
reversed-phase separations with minimal secondary interactions.
The
use of a 3 μm silica particle reduces limits for diffusion of the
analytes into the stationary phase, resulting in fast, high-resolution
separations. Compared to 5 μm column packings, the smaller particles
support increased flow rates and shallower gradients on shorter columns,
for faster separations. Dionex tests each bonded silica lot for peptide
selectivity to ensure reproducible performance.
Acclaim PepMap for TFA-Free Peptide Mapping Specifications
|
Column type
|
Nano
|
Capillary
|
Micro
|
Column i.d.
|
75 μm
|
300 μm
|
1.0 mm
|
Length
|
50, 150, 250, and 500 mm
|
50, 150, and
250 mm
|
50, 150, and
250 mm
|
Recommended Flow Rate
|
200 nL/min
|
4 μL/min
|
40 μL/min
|
Packing Material
|
C18, 100 ?,
3 μm
|
C18, 100 ?,
3 μm
|
C18, 100 ?,
3 μm
|
C18, 300 ?,
5 μm
|
C18, 300 ?,
5 μm
|
C18, 300 ?,
5 μm
|
C8, 100 ?,
3 μm
|
C8, 100 ?,
3 μm
|
C8, 100 ?,
3 μm
|
C8, 100 ?,
5 μm
|
C8, 100 ?,
5 μm
|
C8, 100 ?,
5 μm
|
C4, 300 ?,
5 μm
|
C4, 300 ?,
5 μm
|
C4, 300 ?,
5 μm
|
Column
|
Fused-silica
|
Glass Lined Tubing
|
Stainless steel
|
Column Inlet
|
1/16-in.
PEEK sleeve adapter
(provided)
nanoViper (optional)
|
1/16-in.
universal fitting
nanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings
|
1/16-in.
PEEK tubing
nanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittings
|
Column Outlet
|
Fused-silica
25-μm i.d./ 280-μm o.d.
|
Fused-silica
75-μm i.d./ 280-μm o.d
|
1/16-in.
PEEK tubing
nanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittings
|
MS Outlet
|
1-m Length
|
The
standard particle size is nominally 3 μm. Analytical columns are
available in 2.1, 3.0, and 4.6 mm dimeters; standard lengths are 50 and
150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and
custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in
both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.
Analytical Columns
|
Acclaim 300, C18, 3 μm Analytical (2.1 x 50 mm)
|
060263
|
Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm)
|
060264
|
Acclaim 300, C18, 3 μm (3.0 x 150 mm)
|
063684
|
Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm)
|
060265
|
Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm)
|
060266
|
Guard Columns
|
Acclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580
|
069690
|
PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA
|
069697
|
Hardware
|
Acclaim SST Guard Cartridge Holder V-1
|
059456
|
Guard to Analytical Column Coupler
|
059457
|
Guard Kit (Holder and Coupler)
|
059526
|
Acclaim SST Guard Cartridge Holder V-2
|
069580
|
Acclaim Guard Kit (Holder and coupler) V-2
|
069707
|
Guard to Analytical Column Coupler V-2
|
074188
|
Micro and Nano Columns
|
Acclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper
|
164567
|
75 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper
|
164708
|
300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper
|
164716
|
300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper
|
164718
|
Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm
|
163584
|
Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper
|
164572
|